miscript rt ii kit (Qiagen)
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miscript rt ii kit
Miscript Rt Ii Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+ii+rt+kit/Reverse+transcriptase/us12447172-220-9-13
Average 99 stars, based on 7641 article reviews
Miscript Rt Ii Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+ii+rt+kit/Reverse+transcriptase/us12447172-220-9-13
Average 99 stars, based on 7641 article reviews
miscript rt ii kit - by Bioz Stars,
2026-11
99/100 stars
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Generated:Article Title: miR-769-5p has diagnostic value in acute kidney injury in intensive care unit patients and mediates disease development by targeting SIRT6. Article Snippet: .. For miRNAs such as miR-769-5p, cDNA was generated with the use of Synthesized:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Article Title: Administration of Antibiotics Downregulates the Expression of MicroRNA-200 Family Members in Lamina Propria T Cells of the Murine Large Intestine. Article Snippet: .. First-strand cDNA was synthesized using the Quantitative RT-PCR:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using SYBR Green Assay:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Article Title: miR-7-5p and Importin-7 Regulate the p53 Dynamics and Stability in Malignant and Benign Thyroid Cells. Article Snippet: Total RNA from cultured cells and paraffin-embedded thyroid tissues was isolated using TRIzol (Thermo Fisher Scientific Inc, Waltham, MA, USA) and miRNeasy FFPE Kit (Qiagen, Hilden, Germany), respectively, following the manufacturers’ instructions. .. Reverse transcription of cDNA and RT-PCR were performed using Polymerase Chain Reaction:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Sequencing:Article Title: Binary curcuminoid complex CRE-Bin inhibits osteoclast differentiation by suppressing the canonical NF-κB signaling pathway and modulating microRNA-223 expression. Article Snippet: The primers used were as follows: CTSK: Forward: 5′- A T G T G G G G G C T C A A G G T T C T G-3′w Reverse: 5′- C A T A T G G G A A A G C A T C T T C A G A G T C-3′ NFI-A: Forward: 5′- T T A C A T G T T G G T T C C C T C A G C-3′ Reverse: 5′- T G C T T A C C T G G G T G A G A C A G-3′ GAPDH: Forward: 5′- A A A T G G T G A A G G T C G G T G T G-3′ Reverse: 5′- G A A T T T G C C G T G A G T G G A G T-3′ miR-223 expression was measured according to Madhyastha et al.26. miRNA-enriched total RNA was extracted using an miRNeasy kit (Qiagen). .. First-strand cDNA was synthesized from 1 μg RNA using Reverse Transcription:Article Title: miR-145 and miR-23b co-transfection decreases proliferation, migration, invasion and protein levels of c-MYC, ZEB1 and ABCB1 in epithelial ovarian cancer cell lines Article Snippet: As a negative control, cells were transfected with a scrambled sequence (Sc; cat. no. SC-37007; Santa Cruz Biotechnology, Inc.) at a final concentration of 10 nM under the aforementioned conditions. .. The Article Title: miR-7-5p and Importin-7 Regulate the p53 Dynamics and Stability in Malignant and Benign Thyroid Cells. Article Snippet: Total RNA from cultured cells and paraffin-embedded thyroid tissues was isolated using TRIzol (Thermo Fisher Scientific Inc, Waltham, MA, USA) and miRNeasy FFPE Kit (Qiagen, Hilden, Germany), respectively, following the manufacturers’ instructions. .. Reverse transcription of cDNA and RT-PCR were performed using Article Title: Micro RNAs as a potential biomarker for predicting liver fibrosis severity in hepatitis C virus affected patients Article Snippet: .. First, reverse transcription was carried out later to form complementary deoxyribonucleic acid (cDNA) employing Reverse Transcription Polymerase Chain Reaction:Article Title: miR-7-5p and Importin-7 Regulate the p53 Dynamics and Stability in Malignant and Benign Thyroid Cells. Article Snippet: Total RNA from cultured cells and paraffin-embedded thyroid tissues was isolated using TRIzol (Thermo Fisher Scientific Inc, Waltham, MA, USA) and miRNeasy FFPE Kit (Qiagen, Hilden, Germany), respectively, following the manufacturers’ instructions. .. Reverse transcription of cDNA and RT-PCR were performed using Real-time Polymerase Chain Reaction:Article Title: miR-7-5p and Importin-7 Regulate the p53 Dynamics and Stability in Malignant and Benign Thyroid Cells. Article Snippet: Total RNA from cultured cells and paraffin-embedded thyroid tissues was isolated using TRIzol (Thermo Fisher Scientific Inc, Waltham, MA, USA) and miRNeasy FFPE Kit (Qiagen, Hilden, Germany), respectively, following the manufacturers’ instructions. .. Reverse transcription of cDNA and RT-PCR were performed using Article Title: Administration of Antibiotics Downregulates the Expression of MicroRNA-200 Family Members in Lamina Propria T Cells of the Murine Large Intestine. Article Snippet: .. First-strand cDNA was synthesized using the Control:Article Title: Administration of Antibiotics Downregulates the Expression of MicroRNA-200 Family Members in Lamina Propria T Cells of the Murine Large Intestine. Article Snippet: .. First-strand cDNA was synthesized using the cDNA Synthesis:Article Title: Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma Article Snippet: RNA integrity and concentration were evaluated with a NanoDrop ND-1000 spectrophotometer, measuring absorbance at 260/280 nm. .. Complementary DNA (cDNA) synthesis was carried out with 1 μg of miRNA using the |